Jan Vacek

Department of Medical Chemistry and Biochemistry

Faculty of Medicine and Dentistry, Palacky University
Hnevotinska 3, CZ-775 15, Olomouc, Czech Republic

Tel.: +420 585 632 303
E-mail address: jan.vacek@upol.cz

VITAE:  J.V. is a professor of medical chemistry and biochemistry at the Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Czech Republic.

RESEARCH TOPICS: redox biochemistry, bioelectrochemistry and biophysical methods for nucleic acids and protein research; sensors and electronics for the study of DNA- and protein-drug interactions; functional polymers and the development of new microelectrode-based approaches. For applications and cooperations with commercial sphere see, www.cb21pharma.com and www.microdialysis.eu

SELECTED PUBLICATIONS:

J. Vacek et al., Electrochemistry in sensing of molecular interactions of proteins and their behavior in an electric field, Microchim. Acta, 190, 442, 2023 (PDF)

M. Zatloukalova et al. , Cubosomal lipid formulation of nitroalkene fatty acids, Redox. Biol. 46, 102097, 2021 (PDF)

J. Vacek et al., Antioxidant function of phytocannabinoids, Free Radic. Biol. Med. 164, 258-270, 2021 (PDF)

M. Zatloukalova et al., Redox properties and human serum albumin binding of nitro-oleic acid, Redox Biol. 24, 101213, 2019 (PDF)

V. Dorcak et al., Structural Stability of Peptidic His-Containing Proton Wire in Solution and in the Adsorbed State, Langmuir, 34, 6997−7005, 2018 (PDF)

M. Svrckova et al., Na/K-ATPase Interaction with Methylglyoxal as Reactive Metabolic Side Product, Free Radic. Biol. Med. 108, 146−154, 2017 (PDF)

J. Vacek et al., Electrochemical Platform for the Detection of Transmembrane Proteins Reconstituted into Liposomes, Anal. Chem. 88, 4548−4556, 2016 (PDF)

M. Havlikova et al., Electrocatalytic Assay for Monitoring Methylglyoxal-mediated Protein Glycation, Anal. Chem. 87, 1757−1763, 2015 (PDF)

Reduced Abstract: Protein glycation is a complex process that plays an important role in diabetes mellitus, aging, and the regulation of protein function in general. As a result, current methodological research on proteins is focused on the development of novel approaches for investigating glycation and the possibility of monitoring its modulation and selective inhibition. In this paper, a first sensing strategy for protein glycation is proposed, based on protein electroactivity measurement. The methodology developed also provides a new option for the analysis of protein intermolecular interactions using electrochemical sensors.

 

RECENT REVIEWS AND MONOGRAPHS:

J. Vacek et al., Redox biology and electrochemistry. Towards evaluation of bioactive electron donors and acceptors, Curr. Opin. Electrochem., 36, 101142, 2022 (PDF)

A. Van Gool et al. Analytical techniques for multiplex analysis of protein biomarkers, Exp. Rev. Proteom. 17, 257-273, 2020 (PDF)

N. Cibicek, J. Vacek (Eds.): Principles and Applications of Analytical Methods in Laboratory Medicine, Palacky University Press (in Czech), ISBN: 978-80-244-3951-8, Olomouc, Czech Republic, 2020 (book)

S. Sek et al., Electrochemistry of peptides, Curr. Opin. Electrochem. 14, 166-172, 2019 (PDF)

J. Vacek et al., Electrochemistry of membrane proteins and protein–lipid assemblies, Curr. Opin. Electrochem. 12, 73–80, 2018 (PDF)

J. Egea et al., European Contribution to the Study of ROS, Redox Biol. 13, 94−162, 2017 (PDF)

J. Vacek et al., New Directions in the Electrochemistry of Biomacromolecules: Short Overview and Discussion, Chem. Listy (in Czech) 111, 430−438, 2017 (PDF)

NEW TECHNOLOGIES AND TECHNOLOGICAL IMPROVEMENT:

INVITED PARTICIPATION ON RECENT CONFERENCES (2022-2023):

J. Vacek: Lipid Membrane Behavior of Nitro-Fatty Acids and their Loading into POPC Liposomes to Activate Nrf2 Pathway, 23rd Joint meeting of Biophysical Chemists and Electrochemists 2023, J. Heyrovský Institute, Prague, Czech Republic.
J. Vacek: Electrochemistry and Chiral Surface-Supported Films, 4th European BioSensor Symposium 2023, Aachen, Germany.
J. Vacek: New Electrochemical Approaches in Redox Biology Research: Bioactive Electrophiles, 18th International Conference on Electroanalysis ESEAC 2020/2022, Vilnius, Lithuania.